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SUMMARYGUIDO,M.C.
Comparative study of uterine-cervical microbiota at female buffaloes and cows
submitted to ovsynch, at artificial insemination and conception rate
relationship.[Estudo
comparativo da microbióta cérvico-uterina de búfalas e vacas leiteiras
no momento da inseminação artificial,submetidas ao tratamento de
“ovsynch” e sua relação com a taxa de concepção].São Paulo, 2000, 69 p.
Tese (Mestrado) – Faculdade de Medicina Veterinária e Zootecnia, Universidade
de São Paulo.
Infections of bovine and buffaloes female reproductive organs are the causes of
a series of disturbs like: repeat breeding, fetal envoltory retention,
endometritis, metritis, cervicitis and others. Aiming to verify the presence of
microorganisms in the uterine-cervical region in the moment of artificial
insemination of bovine and bubaline females, its variation related with
post-partum period, body score of the animals and its influence on the
conception rate; 77 bovines and 87 bubaline with more than 40 days pos-partum
have been studied. Animals have been checked, evaluating its reproductive
conditions and its corporal status that was
scored from 1 to 5 and submitted to ”ovsynch”(GnRH/PGF/GnRH)
treatment. In the moment of artificial insemination, samples of “swab” have
been collected of the cervical uterine region, inoculated in BHI broth medium ,
added with vaseline for posterior identification of
aerobic and anaerobic microorganisms. Among the bovines it was verified a
higher frequency of bacteria isolation (77,9%) than in bubaline (41,4%)
statistically significant ( p
< 0,05). Microbiota isolated from bubaline was 39,5%, Staphylococcus
sp. (Staphylococcus
epidermidis and Staphylococcus
saprophyticus), 31,6%, Bacillus sp,
10,5% , Corynebacterium
sp, 7,9%
Streptococcus bovis,
5,3% Escherichia
coli , others 5,3%. Among bovine
it was isolated 25,4% , Streptococcus sp
(Streptococcus pyogenes
and
Streptococcus
bovis), 25,4% Bacillus
sp, 20,6% Staphylococcus
sp. (Staphylococcus epidermidis and
Staphylococcus saprophyticus ) , 19%
enterobacteriacea ( Escherichia
coli, Salmonella sp , Yersinia
pseudotuberculosis) , 4,8 % Corynebacterium
sp others 4,8%. It was not observed
differences between bovine and bubaline microbiota, except for the Streptococcus
sp that was isolated in a higher frequency in the bovines, with a
statistically significant difference (P<
0,05). It was not detected any influence of the uterine-cervical microbiota in
the conception rate. There was no difference in the frequency of isolation of
microorganisms among the animals with more than 100 days post-partum or less
than 100 days, neither related to the different body scores. It has been
observed a positive correlation between the body scores and
conception rate. Uniterms: Artificial insemination; Microbiota; uterine-cervical swab; Bovine; Buffaloes. |
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